Continuation Part 13: Amanda Knox/Raffaele Sollecito

Once again, the thread has grown lengthy, so this is a continuation from Part 12. For further reference, see also Part 11, Part 10, Part 9, Part 8, Part 7, Part 6, Part 5, Part 4, Part 3, Part 2, and Part 1.

Posted By:Agatha

Quote:

Originally Posted by Planigale (Post 10458051) Stefanoni has got herself into a bit of a double bind. If the quantity was above 100 pg then she had sufficient to run replicates, but she said the amount was too low to run replicates so she obviously thought it was much lower, probably less than 1000 ng (a hundred fold difference). 100 pg would not be LCNDNA, but everyone agreed it was, even Stefanoni never objected to this, so she obviously thought the amount was below 10 pg.

I think your measures are off, but this is a good point: Stefanoni believed that she didn't have enough DNA in 36b to run replicates. But, since the minimum amount that could be detected in the QF is 3ng in the 50 uL sample, there is no way that the QF could provide her with enough data to determine whether she could split a sample (i.e., a 50 uL sample could have had 2ng of DNA in it (plenty to allow a non-LCN split), and yet this would still report as "too low").



Moreover, I see no evidence that Stefanoni ever replicated ANY amplifications, and there are some amplifications that are clearly from LCN samples. She just never did it, it was not her protocol. So, all of this stuff about splitting samples and replications is just a great big Stefanoni lie.